![]() ![]() ![]() These findings have important implications for sugarcane genebank management, germplasm exchange, and crop genetic improvement. The single-dose SNP markers developed in this study offer a robust tool for characterizing sugarcane germplasm worldwide. Analysis of Molecular Variance revealed that within-species genetic variations accounted for 85% of the total molecular variance, with the remaining 15% attributed to among-species genetic variations. Population structure and genetic diversity in these five species were further supported by Principal Coordinate Analysis and neighbor-joining clustering analysis. Moreover, we clarified the species membership and parentage of 298 clones that had ambiguous passport records (e.g., ‘ Saccharum spp’, ‘unknown’, and ‘hybrid’). An assignment test, based on the five Saccharum species, enabled correcting 141 instances of mislabeled species memberships and inaccuracies. Hierarchical analysis of population structure partitioned the Saccharum germplasm into five clusters, corresponding to S. In addition, twenty-seven homonymous groups were detected, which shared the same clone name but differed in SNP genotypes. Specifically, we identified 86 groups of duplicates, characterized by identical SNP genotypes, which encompassed 211 accessions (23% of the total clones), while 135 groups, constituting 471 clones (52% of the total), exhibited near-identical genotypes. Our analysis uncovered a high rate of clone mislabeling in the collection. In this study, we employed a single-dose SNP panel to genotype 901 sugarcane accessions, representing six Saccharum species and various hybrids. germplasm in this collection has not been fully analyzed. However, the genetic integrity of the Saccharum spp. The World Collection of Sugarcane and Related Grasses, maintained at the USDA-ARS in Miami, FL, is one of the largest sugarcane germplasm repositories in the world. 2Subtropical Horticulture Research Station, United States Department of Agriculture, Agriculture Research Service, Miami, FL, United States.1Sustainable Perennial Crops Laboratory, United States Department of Agriculture, Agriculture Research Service, Beltsville, MD, United States.Sunchung Park 1 Dapeng Zhang 1* Gul Shad Ali 2* ![]()
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